Download e-book for kindle: Advances in Enzymology and Related Areas of Molecular by Nord F F (ed)

By Nord F F (ed)

ISBN-10: 0470122757

ISBN-13: 9780470122754

ISBN-10: 0470649593

ISBN-13: 9780470649596

Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative experiences of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unmatched view of the old improvement of enzymology. The sequence deals researchers the newest knowing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic procedure, and their software in either the laboratory and undefined. each one quantity within the sequence beneficial properties contributions via major pioneers and investigators within the box from around the globe. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of issues and lengthy ancient pedigree, Advances in Enzymology and comparable parts of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by means of any scientist drawn to the invention of an enzyme, its homes, and its purposes.

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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 30

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It was further suggested that cyt f and plastocyanin may act in parallel in transferring electrons from photosystem 2 to P-700 in photosystem 1. The results of Gorman and Levine with Chlamydomonas argue against a parallel formulation, but they would be compatible with the existence of a “supercomplex” among P-700, cyt f, and plastocyanin, in which both cyt f and plastocyanin are photooxidizable a t low temperatures. Direct evidence in favor of a “supercomplex” would be provided by the observation of plastocyanin photooxidation a t low temperature, but this has not been reported so far.

Our studies on the fractionation of the photochemical systems and on the composition and properties of the separated particles will now be described in some detail. 44 N. K. BOARDMAN A. PARTICLES OBTAINED BY T H E DIGITONIN PROCEDURE I . 5% digitonin for 30 min a t 0°C. The chlorophyll-containing particles were separated by differential centrifugation a t the following speeds : 1OOOg for 10 min, 10,OOOg for 30 min, 50,OOOg for 30 min, and 144,OOOg for 60 min. The pellets from the centrifugations were resuspended in phosphate buffer and designated lOOOg, lO,OOOg, 5O,OOOg, and 144,0009 fractions.

5yOdigitonin. Centrifugation was for 40 hr at 60,0009. From the top downwards appeared a yellowish-green band (absorption maximum a t 671 mp) due presumably t o solubilized chlorophyll, a light-pink band which contained cytochromes f and b6, and a blue-green band (absorption maximum 678 mp). Plastocyanin was present a t the top of the gradient. The blue-green band was able t o photoreduce NADPf if provided with an electron donor, ascorbate-DCTP, and the enzymes ferredoxin, ferredoxin-NADP reductase, and plastocyanin.

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Advances in Enzymology and Related Areas of Molecular Biology, Volume 30 by Nord F F (ed)


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