By Schloemilch O.
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Additional info for Allgemeine Umkehrung gegebener Funktionen
27. Diagram of microchromatograms (polyamide layers) using the solvent systems (A) methyacetate/isopropanol/ammonia and (B) butanol/chloroform/acetic acid for the fractionation of dopamine (DA), noradrenaline (NA), serotonin (5-HT), 3,4-dihydroxyphenylalanine (DOPA), adrenaline (AD) and 5-hydroxytryptophan (5-HTP). ) TABLE 18. 000 This very simple procedure for the analysis of amines can be applied to a number of neurochemical problems. The applicability of the method is demonstrated by the following experiment.
9 ng of serotonin. 7 ng serotonin per cell. The described procedure is especially suitable for analysing the amine content in neurons thought to contain more than one type of amine and has also been successfully adapted to measure the rate of incorporation of radioactivity into specific neurons in order to measure the biosynthesis and half-lives of certain monoamines. The method has the disadvantage that amounts of proteins which would not interfere with the separation of dansyl derivatives impair the separation of the amines.
1973) Neuhoff £¿ al. (1969) Neuhoff and Weise (1970) Osborne et al. (1971), Briel et al. (1972) area under the density curve, and are expressed as percentages of the sum of the base areas. This technique, with a simple modification (see Edstrõm, 1964), also allows DNA base content and composition to be determined. The fibres normally employed for electrophoresis or microphoresis, as termed by Edstrõm (1956), are made from cellulose or rayon silk. However, for radioactive procedures it is best to use strips made from cellophane (Koenig and Brattgârd, 1963), although the sensitivity of the procedure is slightly decreased since more than 3000pg starting material is required.
Allgemeine Umkehrung gegebener Funktionen by Schloemilch O.